The device serves kakım an inexpensive alternative to a spectrophotometer, which is commonly used for these applications. Because DEN-600 is battery powered and compact, it birey be comfortably located in a biosafety cabinet, anaerobic chamber or quickly moved to another lab room.
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Like any spectrophotometer and colorimeter, a densitometer also uses the principle of photometry. The light from the stabilized source passes through the optical system inside the densitometers. The light absorption depends on the density of the substances. The absorbance is indirectly proportional to the density, i.e., the denser the substance, the lesser the absorbance.
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The densitometer used in a microbiology laboratory is hence named the McFarland densitometer. It is helpful laboratory equipment as an alternative method for preparing McFarland standards of bacteria and fungi cell suspension.
A polishing step is employed to target the removal of unwanted molecule isoforms and other impurities, e.g. use of a cation exchange membrane absorber to remove all positively charged impurities, while leaving the negatively charged target molecule to pass through the media.
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Prefiltration may be carried out using a glass or cellulose based prefilter with a more porous filter matrix to enable a two-step filtration process. Likewise, filter aids such bey pharmaceutical-grade diatomaceous earth kişi be applied directly within the cell culture to enable a prefiltration matrix layer on tamamen of the 0.2µm membrane layer to prevent blockages.
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When there is a need to separate a solid-liquid mixture, on occasion it is possible to pour off the liquid while leaving the solid behind. This process is called decanting, and is the simplest separation method.
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Intensive stirring of bacterial and yeast cells, extraction of metabolites and enzymes from cells and cell cultures, and various DNA operations such kakım deproteinization SRL Sisco of DNA/protein complexes and purification of low molecular weight DNA/RNA fragments in PCR diagnostics.